Download e-book for iPad: Calcium-Binding Protein Protocols: Volume 2: Methods and by Vogel

By Vogel

ISBN-10: 0896036898

ISBN-13: 9780896036895

Blurb for quantity 2 Hans Vogel and a panel of top researchers overview the protein chemistry and behaviour of this important type proteins, and supply a complete choice of confirmed experimental ideas for learning it either in vitro and in vivo. This moment quantity makes a speciality of state-of-the-art experimental ideas for learning the answer constitution, balance, dynamics, calcium-binding houses, and organic task of calcium-binding protein as a rule. as well as enzymatic assays and extra regimen spectroscopic and protein chemistry ideas, there also are NMR techniques, thermodynamic analyses, kinetic measurements corresponding to floor plasmon resonance, concepts for amino acid series alignments, and fluorescence ways to learn the distribution of calcium and calcium-binding proteins in cells. the 1st spouse quantity, experiences and Case Histories units the level for this quantity through introducing many of the periods of intra- and extra-cellular calcium-binding proteins and their mode of motion. unmarried Blurb for either Volumes Hans Vogel and a panel of major researchers evaluate the protein chemistry and behaviour of this important protein type, and supply a complete selection of confirmed experimental ideas for his or her research either in vitro and in vivo. the 1st quantity discusses the function of calcium in intracellular secondary messenger activation mechanisms, together with designated points of calcium chemistry and its usage in dairy proteins and blood clotting. specified case reports supply a wealth of important information regarding protein purification and characterization thoughts, X-ray crystallography, and particular calcium-binding proteins and their modes of motion. the second one quantity specializes in state of the art experimental strategies for learning the answer constitution, balance, dynamics, calcium-binding homes, and organic job of calcium-binding protein quite often. as well as enzymatic assays and extra regimen spectroscopic and protein chemistry options, there also are NMR methods, thermodynamic analyses, kinetic measurements similar to floor plasmon resonance, innovations for amino acid series alignments, and fluorescence how to research the distribution of calcium and calcium-binding proteins in cells.

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Additional resources for Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology Vol 173)

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4. Take out the dialysis cell from the incubator, place it on the bench, and discharge the solvent in the lower chamber into the bottle for waste 45Ca2+. 5. Disassemble the apparatus carefully with releasing screws. With tweezers, put the upper and lower chambers and stirring bars into the detergent solution, and the dialysis membrane into the can for the radioactive waste. 6. Wash the disassembled parts thoroughly with detergent solution, rinse with the distilled water. 4. Calculation to Make a Ca2+-Binding Curve 1.

Chem. 250, 4628 –4633. 2. Crouch, T. H. and Klee, C. B. (1980) Positive cooperative binding of calcium to bovine brain calmodulin. Biochemistry 19, 3692–3698. 3. Teraoka, H. and Nierhaus, K. H. (1979) Measurement of the binding of antibiotics to ribosomal particles by means of equilibrium dialysis. Methods Enzymol. 59, 862 –866. 4. Colowick, S. P. and Womack, F. C. (1969) Binding of diffusible molecules by macromolecules: rapid measurement by rate of dialysis. J. Biol. Chem. 244, 774–777. 5. Womack, F.

T. (1977) Partial purification of the Ca2+-Mg2+ ATPase activator from human erythrocytes: its similarity to the activator of 3':5'- cyclic nucleotide phosphodiesterase. Biochem. Biophys. Res. Commun. 77, 1210–1216. 28. Schulman, H. and Greengard, P. (1978) Stimulation of brain membrane protein phosphorylation by calcium and an endogenous heat-stable protein. Nature 271, 478 –479. 40 Haiech and Kilhoffer 29. Stewart, A. , Ingebritsen, T. , Klee, C. , and Cohen, P. (1982) Discovery of a Ca2+- and calmodulin-dependent protein phosphatase: probable identity with calcineurin (CaM-BP80).

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Calcium-Binding Protein Protocols: Volume 2: Methods and Techniques (Methods in Molecular Biology Vol 173) by Vogel


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