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1. Finally, when the column is overloaded the peak height does not increase linearly with sample size but peak area does, at least if the end of the peak can be properly detected. Also at low sample size a significant part of some components may be quasi-irreversibly adsorbed on the packing material. 4 Detector Selectivity Most detectors have response factors which vary widely from one component to another. The selectivity characterizes this variation in response with the chemical nature of the compounds studied.
For example the detector cell volume should be less than 1 Ill. This is difficult to achieve with a UV detector without some reduction in the optical path length, hence an increase in the detection limits which is opposite to the rationale in using microbore columns. The specifications can be relaxed somewhat by chosing columns longer than the conventional ones and operating then at a higher flow velocity to a comparable analysis time. But then the inlet pressure is large, the dilution is increased which again is not satisfactory.
The variance contribution of short tubes is small if they are well designed 15) and will thus be neglected here. The variance contribution of the detector can be measured directly using a very fast sampling system and a narrow, short capillary tube in place of the column I6 ). It depends markedly on the flow-rate, being controlled at low flow rate by mass transfer processes and at high flow-rates by the response time. The influence of these factors has been studied in details 17 - 19). The time constant, <, of a detector describes the speed at which it responds to an instantaneous change of concentration of solute in the detector cell.
Data Processing Equipment Encyclopedia Vol 2 - electronic devices - Gille Assoc.